Hemolysin production, and adhesion related traits such as adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulent attributes associated in pathogenicity of Candida. Post-antifungal effect (PAFE), which is the suppression of fungal growth following brief exposure to antifungal agents, also have an impact on pathogenicity and virulence of Candida. Candida dubliniensis is associated with both oral and systemic candidosis, which can be managed with caspofungin (an echinocandin antifungal drug). There is no information on caspofungin-induced PAFE and its impact on hemolysin production and adhesion related traits of Candida dubliniensis isolates. Therefore, the main aim of this study was to determine the in vitro PAFE on 20 Candida dubliniensis isolates following brief exposure to caspofungin. Furthermore, the impact of caspofungin-induced PAFE on hemolysin production and adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, Candida dubliniensis isolates were exposed to sub-lethal concentrations (×3 MIC) of caspofungin for 1 hour (i.e. brief exposure). Thereafter the drug was removed by dilution and the PAFE, hemolysin production, adhesion to BEC and DAS, GT formation and CSH was determined by previously described in-vitro assays. MIC (µg/ml) of Candida dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE (hours) on Candida dubliniensis isolates was 2.17. Brief exposure to caspofungin suppressed the ability of Candida dubliniensis isolates to produce hemolysin, adhere to BEC and DAS, GT formation and CSH by a mean percentage reduction of 17.09%, 69.97%, 71.95%, 90.06% and 32.29% (p<0.001 for all), respectively. Therefore, it was observed that brief exposure of Candida dubliniensis isolates to caspofungin would produce an antifungal effect not only by suppressing its growth, inducing a PAFE, but also by altering candidal hemolysin production and adhesion related traits of this clinically important fungal pathogen. The work was supported by Kuwait University Research Grant No. DB 01/14.