This retrospective study was conducted at different private hospital, Dhaka during July to December, 2015 with a view to find out incidence of UTI patients with aiming to testing antimicrobial drug susceptibility.In order to conduct this study, consecutive urine samples of 400 of total population of both sexes and various age groups were taken in a hospital setting of both out patients and in patients. There was marked gender variations is all age groups, which comprised 0-90 years of age of the member of urine sample requested for examination only 36.75% of specimens yielded positive culture. UTI is more prevalent in female then in male.The most common isolates in the Gram negative organisms were E.coli(31.11%),Enterococcus spp.(18.51%),Klebsiella spp.(9.62%)Streptococcus spp. Streptococcus agalactia(8.14%)Staphylococcus aureus(3.70%),Staphylococcus haemolyticus(3.70%) Candida(1%)and pseudomanas(1.48%), Proteus sp. (2.96%), Acinetobacter spp.(2.20%)Serratia spp.(1.48%), Prevalence of UTI cases was caused by 67.91% of Gram negative & 32.08 of Grams positive organisms.The study also reveals that patients those are UTI cases are associated with abnormal count of pus cells, albumin and sugar in most cases.This study showed that organisms are resistant to common urinary antibiotics which were used before through oral route, such as Cotrimoxazole, Nitrofurantion, Nalidixic Acid. Now E.colis sensitive to colistin (Polimyxin E) and carbepenem ranging from 82-89% followed by Amikacin and Nitrfurantion from 63-72%. On the other hand highly resistances were revealed against Cefepime and Nalidixic acid from 63-72%. Also found resistance against Ceftriaxone (61%), Ceftazidime (59%), Cotrimoxazole (57%).The study revealed that the intravenous antibiotic option is more than oral route.