Actinobacillus pleuropneumoniae (APP), a major respiratory pathogen of pigs, causes dramatic losses in the farming industry every year. The majority of methods for studying airborne pathogens involve impacting air directly onto solid media. This method can fail to detect fastidious pathogens, as non-significant contaminants can overcrowd the plates. This study presents a method for collection of airborne pathogens utilising the Coriolis™ system. Particulates present in the air are collected though centrifugal force into a liquid medium, which can then be concentrated or diluted as necessary and used for directly plating onto a variety of media and in culture independent tests such as polymerase chain reaction (PCR) assays. For specific testing for A. pleuropneumoniae, a real time quantitative polymerase chain reaction (qPCR) assay was developed and shown to be capable of detecting down to 10 copies in a sample. This method of environmental testing was used to survey a commercial farm over a period of six months. Samples were collected from pigs of one day of age to 18 weeks. The qPCR results showed that air taken from sheds containing pigs 8, 12, 16 and 18 weeks of age was positive for the presence of APP. Although no clinical signs were seen within these populations, the presence of APP in the environment is indicative of subclinical infection. As A. pleuropneumoniae has the potential to cause large scale and rapid loss, a reliable method for screening for this pathogen would be beneficial to the pig industry.