Shiga toxin-producing E. coli (STEC) O157:H7 are lethal foodborne pathogens frequently isolated from raw beef. The use of phages as antibacterial agents to control bacterial contamination in meat has been described recently. Here, we report the isolation, characterization and application of a lytic phage for controlling STEC O157:H7. The phage PE37, a member of Myoviridae family with a broad host range and linear double stranded genomic DNA molecule, was isolated from bovine intestine sample. The phage was stable under various pH and temperature and significantly reduced the viable counts of STEC O157:H7 by 4.15 and 1.57 log CFU/ml at 6 h incubation in broth at 37°C and 8°C, respectively. The treatment of raw beef artificially inoculated with STEC O157:H7 at 5 x 105 CFU/ 4 cm2 meat piece by phage PE37 at 25°C and 8°C for 24 h reduced bacterial viable counts by 2.33 and 0.93 log, respectively, compared to non-treatment. The addition of PE37 to the raw beef samples contaminated with mixture of STEC O157:H7 and extended-spectrum beta lactamase-producing E. coli isolates also decreased the bacterial viable counts by 1.36 and 1.04 log at 25°C and 8°C, respectively, compared to the samples without phage. These findings suggest that phage PE37 is a potential candidate for controlling STEC O157:H7 contamination in meat.