As a top ten most common isolated nosocomial pathogens in tertiary hospital, Stenotrophomonas maltophilia gains attention due to its intrinsically multiple drugs resistance characteristic. It shows wide range of antimicrobial resistance including beta-lactams, cephalosporins, carbapenems, aminoglycosides, and quinolones. Mechanisms involved in drugs resistance are due to its low permeability cell membrane, presence of efflux pumps, and production of antibiotics hydrolyzing enzymes. On the other hand, S. maltophilia shows its agricultural economic value by enhancing the crops’ growth. It is found to live naturally in the rhizosphere to stimulate the plant’s roots and shoots development. Thus, herbicides exposure is inevitable since the chemicals are applied by most of the farmers to assist the crops yield by removing the unwanted weeds. Interestingly, the antimicrobial resistance properties of this bacterium as mentioned above may play an important role to enable it to survive under such high toxic environment. Capability of S. maltophilia to degrade both selective herbicides, dicamba and atrazine, was reported. As a result, dicamba mono-oxygenase (dmo) gene in S. maltophilia was characterized and cloned to develop soybean with dicamba-resistant property. Invention of dicamba-resistant soybean could ease the producer in weeds management and to increase the yield as well. However, the herbicides mentioned above are less common in Malaysia. Thereby, a study was carried out to evaluate the non-selective herbicide-tolerance level of S. maltophilia. Paraquat (Gramazone), glufosinate (Bayer Basta), and glyphosate (Monsanto) are the most common non-selective herbicides used in Malaysia. They kill the plants which they come into contact. Surprisingly, the environmental S. maltophilia isolate showed greater tolerance level to these three types of herbicides compared to clinical isolates. This finding postulates the value of environmental S. maltophilia to be applied in bioremediation of non-selective herbicides residue as well as development of crops with non-selective herbicides tolerant property.