Oral Presentation Australian Society for Microbiology Annual Scientific Meeting 2016

Small Molecules for Treatment of Critical Infectious Diseases (#1)

Ulrike Holzgrabe 1 , Florian Seufert 1 , Jan Glaser 1 , Georg Hiltensperger 1
  1. Institute of Pharmacy, University of Würzburg, Am Hubland, 97470, Würzburg, Germany

Recently, national and international health authorities have declare war on multiresistant bacteria. Beside the growing problem of resistances, a lot of bacterial infections cannot be sufficiently treated because no appropriate anti-infectives are available. Among them are protozoal diseases such as sleeping sickness and leishmaniosis, as well as infections by gram-negative bacteria like Legionella and Burkholderia.

Within the frame of the DFG-funded collaborative research center 630, dealing mainly with tropical infectious diseases, we have developed new quinolone amides which are able to cure Trypanosoma brucei infected mice.[[i]]

Extracts from Valeriana walichii were found to have antileishmanial activity. Bioassay-guided fractionation lead to a caffeic acid bornyl ester of high activity combined with cytotoxicity. The synthesis of a small library of systematically varied compounds result in drugs which were able to cure mice infected with either Leishmania major or Leishmania donovani.[[ii]]

The macrophage infectivity protein (Mip) is a virulence factor of gram-negative bacteria, such as Legionalla, Burkholderia, Francisella, and Yersinia which is often involved in the penetration and/or dissemination of these bacteria. The Mip consist of a peptidyl-proplyl-isomerase whose inhibition result in a reduced cell death in e.g. Burkholderia infected macrophages. Pipecolic esters were found to be potent inhibitors which could be optimized by means of synthesis, X-ray analysis of the ligand-bound Mip and molecular modelling.[[iii]] The compounds show low or none cytotoxicity, fulfill the “Lipinski Rule of Five”, and are, thus, tested in vivo currently.

 

  1. G. Hiltensperger, N. Hecht, M. Kaiser, J.-C. Rybak, A. Hoerst, N. Dannenbauer, K. Müller-Buschbaum, H. Bruhn, H. Esch, L. Lehmann, L. Meinel, U. Holzgrabe, Antimcrob. Agents Chemother. In Revision
  2. J. Glaser, M. Schultheis, S. Hazra, B. Hazra, H. Moll, U. Schurigt, U. Holzgrabe, Molecules, 2014, 19, 1394-1410; J. Glaser, M. Schultheis, H. Moll, B. Hazra, U. Holzgrabe, Molecules, 2015, 20, 5740-53; A. Masic, A. M. Valencia Hernandez, S. Hazra, J. Glaser, U. Holzgrabe, B. Hazra, U. Schurigt, PloS One, 2015, 10(11) e0142386.
  3. D. Begley, D. Fox, Do. Jenner, C. Juli, P. Pierce, J. Abendroth, M. Muruthi, K. Safford, V. Anderson, K. Atkins, S. Barnes, S. Moen, A. Raymond, R. Stacy, P. Myler, B. Staker, N. Harmer, I. Norville, U. Holzgrabe, M. Sarkar-Tyson, D. Lorimer, Antimicrob. Agents Chemother. 2014, 58, 1458-1467.